| First Author | Landau NR | Year | 1984 |
| Journal | Proc Natl Acad Sci U S A | Volume | 81 |
| Issue | 18 | Pages | 5836-40 |
| PubMed ID | 6091113 | Mgi Jnum | J:22043 |
| Mgi Id | MGI:69936 | Doi | 10.1073/pnas.81.18.5836 |
| Citation | Landau NR, et al. (1984) Cloning of terminal transferase cDNA by antibody screening. Proc Natl Acad Sci U S A 81(18):5836-40 |
| abstractText | A cDNA library was prepared from a terminal deoxynucleotidyltransferase-containing thymoma in the lambda phage vector lambda gt11. By screening plaques with anti-terminal transferase antibody, positive clones were identified of which some had beta-galactosidase-cDNA fusion proteins identifiable after electrophoretic fractionation by immunoblotting with anti-terminal transferase antibody. The predominant class of cross-hybridizing clones was determined to represent cDNA for terminal transferase by showing that one representative clone hybridized to a 2200-nucleotide mRNA in close-matched enzyme-positive but not to enzyme-negative cells and that the cDNA selected a mRNA that translated to give a protein of the size and antigenic characteristics of terminal transferase. Only a small amount of genomic DNA hybridized to the longest available clone, indicating that the sequence is virtually unique in the mouse genome. |
