| First Author | Disteche CM | Year | 1985 |
| Journal | Cytogenet Cell Genet | Volume | 39 |
| Issue | 4 | Pages | 262-8 |
| PubMed ID | 2932308 | Mgi Jnum | J:8059 |
| Mgi Id | MGI:56528 | Doi | 10.1159/000132155 |
| Citation | Disteche CM, et al. (1985) Isolation and characterization of two repetitive DNA fragments located near the centromere of the mouse X chromosome. Cytogenet Cell Genet 39(4):262-8 |
| abstractText | Two repetitive DNA fragments located on the mouse X chromosome are described. The fragments were isolated from a lambda phage library enriched in X-chromosomal sequences by flow sorting. Both fragments, which are repeated 20 to 50 times in the genome, were mapped to the mouse X chromosome by Southern blot hybridization to DNA from hybrid cells retaining the mouse X chromosome, by dosage analysis, and by in situ hybridization to mouse chromosomes. In mouse strain C57BL/10BK, one fragment appeared to be located only on the X chromosome, while the other fragment had homologous sequences on chromosome 11 in addition to the X chromosome. The latter fragment showed DNA variants between mouse strains, which are potentially useful for mapping. Both fragments cross-hybridized to another mouse species: Mus caroli. In this species, each fragment appeared to be located on the X chromosome, indicating that some X-chromosome repetitive sequences are partially conserved. In addition, one fragment cross-hybridized to human DNA. |
