| First Author | Pragnell M | Year | 1991 |
| Journal | FEBS Lett | Volume | 291 |
| Issue | 2 | Pages | 253-8 |
| PubMed ID | 1657644 | Mgi Jnum | J:24200 |
| Mgi Id | MGI:72849 | Doi | 10.1016/0014-5793(91)81296-k |
| Citation | Pragnell M, et al. (1991) Cloning and tissue-specific expression of the brain calcium channel beta-subunit. FEBS Lett 291(2):253-8 |
| abstractText | A cDNA clone encoding a protein with high homology to the beta-subunit of the rabbit skeletal muscle dihydropyridine-sensitive calcium channel was isolated from a rat brain cDNA library. This rat brain beta-subunit cDNA hybridizes to a 3.4 kb message that is expressed in high levels in the cerebral hemispheres and hippocampus but is significantly reduced in cerebellum. The open reading frame encodes 597 amino acids with a predicted mass of 65 679 Da which is 82% homologous with the skeletal muscle beta-subunit. The brain cDNA encodes a unique 153 amino acid C-terminus and predicts the absence of a muscle-specific 50 amino acid internal segment. It also encodes numerous consensus phosphorylation sites suggesting a role in calcium channel regulation. The corresponding human beta-subunit gene was localized to chromosome 17. Hence the encoded brain beta-subunit, which has a primary structure highly similar to its isoform in skeletal muscle, may have a comparable role as an integral regulatory component of a neuronal calcium channel. |
