| First Author | Verma M | Year | 1995 |
| Journal | Gene | Volume | 154 |
| Issue | 2 | Pages | 255-7 |
| PubMed ID | 7890173 | Mgi Jnum | J:23967 |
| Mgi Id | MGI:71761 | Doi | 10.1016/0378-1119(94)00807-5 |
| Citation | Verma M, et al. (1995) Primary structure of the canine U1 snRNA. Gene 154(2):255-7 |
| abstractText | The nucleotide (nt) sequence of the canine U1 snRNA was determined. It exhibited significant homology (90-98%) with known U1 sequences. The RNA can be folded according to the secondary structure previously proposed for the U1 snRNA. It contained the conserved sequence UUACCUG in loop A (nt 6-12), required for the recognition of the 5' splice site, and the sequence UGCACU in loop B (nt 68-73), required for recognition of the U1-70K protein. The U1 snRNA was localized in the nucleus and its transcription was sensitive to alpha-amanitin, suggesting that it is transcribed by RNA polymerase II. Southern analysis revealed that the canine genome possesses 5-10 copies of U1 snRNA-encoding genes. |
