| First Author | Vara Prasad MV | Year | 1994 |
| Journal | Oncogene | Volume | 9 |
| Issue | 8 | Pages | 2425-9 |
| PubMed ID | 8036026 | Mgi Jnum | J:19425 |
| Mgi Id | MGI:67595 | Citation | Vara Prasad MV, et al. (1994) Activated mutant of G alpha 13 induces Egr-1, c-fos, and transformation in NIH 3T3 cells. Oncogene 9(8):2425-9 |
| abstractText | Expression of the constitutively activated mutant alpha-subunit of the heterotrimeric G protein G alpha 13 (alpha 13Q226L) leads to the transformation of NIH-3T3 cells. An analysis of the mitogenic pathway mediated by alpha 13Q226L indicated that the expression of the primary response genes, early growth response gene-1 (Egr-1, a nuclear transcription factor with zinc-finger motif) and c-fos (a leucine zipper transcription factor as well as a protooncogene) are constitutively activated in alpha 13Q226L-transformants. While ras-transformed cells did not express Egr-1, cells transformed by the GTPase deficient mutant alpha-subunit of G alpha 12 (alpha 12Q229L) exhibited a weak expression, suggesting that the induction of Egr-1 and c-fos is intrinsic to G alpha 13 signaling pathway and not a consequence of the transformed phenotype. Taken together, these results provide the first evidence that the G alpha 13 signaling pathway involves the activation of specific transcription factors and defines the expression of these nuclear transcription factors as a possible molecular mechanism in alpha 13Q226L-mediated cell proliferation and transformation. |
