| First Author | Fujimoto N | Year | 1995 |
| Journal | J Immunol Methods | Volume | 187 |
| Issue | 1 | Pages | 33-9 |
| PubMed ID | 7490456 | Mgi Jnum | J:29863 |
| Mgi Id | MGI:77387 | Doi | 10.1016/0022-1759(95)00164-6 |
| Citation | Fujimoto N, et al. (1995) Determination of tissue inhibitor of metalloproteinases-2 (TIMP-2) in experimental animals using monoclonal antibodies against TIMP-2-specific oligopeptides. J Immunol Methods 187(1):33-9 |
| abstractText | We have developed a one-step sandwich enzyme immunoassay (EIA) using monoclonal antibodies against oligopeptides of the human tissue inhibitor of metalloproteinases-2 (TIMP-2) (Fujimoto et al. (1993) Clin. Chim. Acta 220, 31). The present studies further demonstrated that the antibodies cross-react with TIMP-2 species of experimental animals including mouse, rat, guinea pig and rabbit. The detection of the TIMP-2 species in our EIA system was verified using rat TIMP-2 and the EIA was subsequently used to measure the animal TIMP-2 in the sera. Using human TIMP-2 as a standard, TIMP-2 levels in the sera of mouse, rat, guinea pig and rabbit were approximately 80, 200, 270 and 25 ng/ml, respectively. |
