| First Author | Bloomquist BT | Year | 1998 |
| Journal | Biochem Biophys Res Commun | Volume | 243 |
| Issue | 2 | Pages | 474-9 |
| PubMed ID | 9480833 | Mgi Jnum | J:45994 |
| Mgi Id | MGI:1196797 | Doi | 10.1006/bbrc.1998.8133 |
| Citation | Bloomquist BT, et al. (1998) Cloning and expression of the human galanin receptor GalR2. Biochem Biophys Res Commun 243(2):474-9 |
| abstractText | Galanin is a peptide hormone which modulates a wide variety of physiological processes, including secretion, muscle contraction, cognitive function, the reproductive axis, and feeding. Two galanin receptor subtypes, GalR1 and GalR2, have been cloned; however, for GalR2 only the rat sequence has been reported in the literature. Our cloning of human GalR2 reveals its amino acid sequence to be 85% identical to rat GalR2 and 39% identical to human GalR1. Binding of [125I]galanin to the human GalR2 receptor transiently expressed in COS-7 cells was saturable (Kd = 0.24 nM +/- 0.06 nM) with a receptor density of 383 +/- 66 fmol/mg protein. Human galanin(1-30) bound with high affinity to the human GalR2 receptor, with a Ki value of 0.86 +/- 0.12 nM. With the identification of a second galanin receptor subtype, the specific functions of human galanin receptor subtypes can now begin to be addressed. |
