| First Author | Allen JW | Year | 1996 |
| Journal | Chromosoma | Volume | 104 |
| Issue | 6 | Pages | 414-21 |
| PubMed ID | 8601336 | Mgi Jnum | J:32446 |
| Mgi Id | MGI:79942 | Doi | 10.1007/BF00352265 |
| Citation | Allen JW, et al. (1996) HSP70-2 is part of the synaptonemal complex in mouse and hamster spermatocytes. Chromosoma 104(6):414-21 |
| abstractText | Mouse spermatogenic cells are known to express HSP70-2, a member of the HSP70 family of heat-shock proteins. The purpose of the present study was to characterize further the expression and localization of HSP70-2 in meiotic cells of mice and hamsters. After separating mouse spermatogenic cells into cytoplasmic and nuclear fractions, proteins were separated by two-dimensional gel electrophoresis and detected with HSP-specific antibodies. Of several HSP70 proteins identified in the cytoplasm, only HSC70 and HSP70-2 were also detected in the nucleus. Immunocytological analyses of spermatocyte prophase cells revealed that HSP70-2 was associated with the synaptonemal complex. Surface-spread synaptonemal complexes at pachytene and diplotene stages labeled distinctly with the antiserum to HSP70-2. Synaptonemal complexes from fetal mouse oocytes failed to show any evidence of HSP70-2. Reverse-transcriptase-polymerase chain reaction (RT-PCR) analyses of gene expression confirmed this sex specificity; Hsp70-2 mRNA was detected in mouse testes, but not ovaries. These findings are suggestive of a previously unsuspected sexual dimorphism in structure and/or function of the synaptonemal complex. |
