| First Author | Kure R | Year | 1996 |
| Journal | Neurochem Res | Volume | 21 |
| Issue | 6 | Pages | 713-22 |
| PubMed ID | 8829145 | Mgi Jnum | J:35321 |
| Mgi Id | MGI:82771 | Doi | 10.1007/BF02527730 |
| Citation | Kure R, et al. (1996) Characterization of DNase I hypersensitive sites in the mouse 68-kDa neurofilament gene. Neurochem Res 21(6):713-22 |
| abstractText | Four brain-specific DNase I hypersensitive sites (HSS) have previously been identified flanking the mouse 68-kDa neurofilament gene within a 1.7 kb upstream sequence which confers neuronal specificity of expression of this gene in transgenic mice. Previously several DNA-binding factors were detected at the HSS closest to the transcription start site (HSS1). However, no major brain-specific factors were identified, suggesting a possible role for the three remaining HSS in conferring tissue-specificity to the NF-L gene. Sequence analysis of the NF-L promoter region demonstrated the presence of an extensive CT repeat and several potential binding sites which are also found in other neurofilament promoters. Gel mobility shift assays revealed a similar but not identical banding pattern with brain and liver nuclear extracts at HSS2, and HSS3, however the banding pattern for HSS4 was predominantly brain-specific. DNase I footprinting revealed several factors binding to the upstream HSS regions in brain and liver nuclear extracts. These include a CCAAT box at HSS2, a novel brain-specific footprint near an adenovirus promoter element E2aE-C beta and a single liver-specific footprint associated with an POU/octamer binding site at HSS4. The presence of brain-specific gel shift bands and tissue-specific footprints associated with HSS4, suggest that this region may play an important role in the regulation of the NF-L gene. |
