| First Author | Yasumoto K | Year | 1997 |
| Journal | Biochim Biophys Acta | Volume | 1353 |
| Issue | 1 | Pages | 23-31 |
| PubMed ID | 9256061 | Mgi Jnum | J:42217 |
| Mgi Id | MGI:1095355 | Doi | 10.1016/s0167-4781(97)00034-1 |
| Citation | Yasumoto K, et al. (1997) Molecular cloning of cDNA encoding a human TFEC isoform, a newly identified transcriptional regulator. Biochim Biophys Acta 1353(1):23-31 |
| abstractText | TFEC is a transcriptional repressor originally identified in rat chondrosarcoma and contains a basic helix-loop-helix and leucine zipper (bHLH/LZ) structure. TFEC shares a closely related bHLH/LZ structure with microphthalmia-associated transcription factor (MITF) and TFE3. In the course of cDNA cloning for a factor structurally related to MITF which is also a regulator for cell differentiation, we have isolated cDNA clones from a THP-1 human monocytic leukemia cell line. These cDNAs encode a protein of 347 amino acids, termed TFECL, a human homolog of a putative rat TFEC isoform. TFECL contains an acidic domain that corresponds to a transcriptional activation domain of TFE3 but its equivalent region is deleted in rat TFEC. We explored a function of TFECL using a melanocyte-specific tyrosinase gene and a ubiquitously expressed heme oxygenase-1 gene, each promoter containing the cis-acting CANNTG motifs. By transient coexpression assays, we showed that TFECL is able to activate or inhibit transcription of a reporter gene linked to either the tyrosinase or the heme oxygenase-1 gene promoter, depending on cell types. These results suggest that TFECL may function as a transcriptional activator under certain conditions. |
