| First Author | Raghunathan K | Year | 1999 |
| Journal | Biochem Pharmacol | Volume | 58 |
| Issue | 5 | Pages | 835-9 |
| PubMed ID | 10449194 | Mgi Jnum | J:56700 |
| Mgi Id | MGI:1342194 | Doi | 10.1016/s0006-2952(99)00157-4 |
| Citation | Raghunathan K, et al. (1999) Modulation of fluorouracil antitumor activity by folic acid in a murine model system. Biochem Pharmacol 58(5):835-9 |
| abstractText | The biochemical basis for modulation of fluorouracil (FU) activity by leucovorin is elevation of the metabolite methylenetetrahydrofolate, which stabilizes the inhibitory ternary complex formed between thymidylate synthase and the active metabolite of FU, 5-fluorodeoxyuridylate. Folic acid, because it can also potentially be metabolized to methylenetetrahydrofolate, was evaluated for its ability to potentiate FU antitumor activity in a dietary folic acid restricted murine model. The plasma pharmacokinetics and tissue distribution of folic acid and all stable metabolites thereof were determined in the model to establish administration schedules. FU was administered to mice implanted subcutaneously with a mammary adenocarcinoma 4 hr after folic acid administration, when the metabolites, methylenetetrahydrofolate and tetrahydrofolate, were elevated maximally in both plasma and tumor tissue. While FU alone suppressed growth 25%, folic acid in combination with FU increased growth suppression to over 70%. These results indicate that folic acid is a potent modulator of FU activity and could be considered as an alternative to leucovorin in the clinical setting. |
