| First Author | Kinnon S | Year | 1999 |
| Journal | DNA Seq | Volume | 10 |
| Issue | 4-5 | Pages | 335-8 |
| PubMed ID | 10727088 | Mgi Jnum | J:67621 |
| Mgi Id | MGI:1930927 | Doi | 10.3109/10425179909033960 |
| Citation | Kinnon S, et al. (1999) Nucleotide sequence of the feline Bmi-1 coding region. DNA Seq 10(4-5):335-8 |
| abstractText | The bmi-1 gene was discovered as a clonal integration site of the Moloney murine leukaemia virus in B-cell lymphomas. The Bmi-1 protein contains the RING finger motif and is homologous to two Drosophila proteins known to be part of a multimeric protein complex involved in repressing gene transcription. A similar role for the highly conserved Bmi-1 protein in mammalian cells has been suggested. The coding regions for the mouse and human genes are known and are 92% homologous. This study involved PCR amplification, cloning, and sequencing of the 980bp feline bmi-1 coding region which was shown to be 92% and 97% homologous to the mouse and human genes respectively. From the open reading frame the feline protein is 326 amino acids in length and is 99% homologous to the human protein and 97% homologous to the mouse protein. This data is consistent with the closer relationship between the feline and human genomes and provides another experimental system in which to analyse Bmi-1 function. |
