| First Author | Stevens S | Year | 2000 |
| Journal | J Immunol | Volume | 164 |
| Issue | 12 | Pages | 6372-9 |
| PubMed ID | 10843692 | Mgi Jnum | J:67813 |
| Mgi Id | MGI:1931429 | Doi | 10.4049/jimmunol.164.12.6372 |
| Citation | Stevens S, et al. (2000) Functional analysis of the OCA-B promoter. J Immunol 164(12):6372-9 |
| abstractText | OCA-B was identified as a B cell-specific coactivator that functions with either Oct-1 or Oct-2 to mediate efficient cell type-specific transcription via the octamer site (ATGCAAAT) both in vivo and in vitro. Mice lacking OCA-B exhibit normal Ag-independent B cell maturation. In contrast, Ag-dependent functions, including production of secondary Ig isotypes and germinal center formation, are greatly affected. To better understand OCA-B expression and, ultimately, the defects observed in the OCA-B knockout mice, we have cloned the OCA-B promoter and examined its function in both transformed and primary B cells. We show here that the OCA-B promoter is developmentally regulated, with activity increasing throughout B cell differentiation. Through physical and functional assays, we have found an activating transcription factor/cAMP response element binding protein binding site (or cAMP response element) that is crucial for OCA-B promoter activity. Furthermore, we demonstrate that IL-4 and anti-CD40 induce both the OCA-B promoter and octamer-dependent promoters, thus implicating OCA-B in B cell signaling events in the nucleus. |
