| First Author | Cyr M | Year | 2001 |
| Journal | Neuroendocrinology | Volume | 73 |
| Issue | 1 | Pages | 37-45 |
| PubMed ID | 11174015 | Mgi Jnum | J:67528 |
| Mgi Id | MGI:1930778 | Doi | 10.1159/000054618 |
| Citation | Cyr M, et al. (2001) Central 5-hydroxytryptamine-2a receptor expression in transgenic mice bearing a glucocorticoid receptor antisense. Neuroendocrinology 73(1):37-45 |
| abstractText | Transgenic mice bearing a transgene coding for a glucocorticoid receptor antisense mRNA that partially blocks glucocorticoid receptor expression were used to investigate the long-term effect of hypothalamic-pituitary-adrenal dysfunction on brain 5-hydroxytryptamine-2A (5-HT(2A)) receptor expression. The brain 5-HT(2A) receptor mRNA levels in transgenic mice were measured by in situ hybridization and compared to those in control mice. We also studied the effect of a 3-week treatment with fluoxetine on brain 5-HT(2A) receptor expression in the transgenic mice. No difference in 5-HT(2A) mRNA levels was observed between transgenic and control mice in cortical or striatal regions, and fluoxetine treatment was without effect. No difference in hypothalamic 5-HT(2A) mRNA levels was observed between transgenic and control mice, while fluoxetine treatment increased these levels in both transgenic as well as in the hypothalamic ventromedial and paraventricular nuclei of control mice. 5-HT(2A) receptor mRNA levels were similar in hippocampal CA1 and CA2 subregions of control and transgenic, but were lower in the CA3 and CA4 subregions of transgenic mice. Fluoxetine had no effect on 5-HT(2A) mRNA levels of transgenic mice but reduced control mouse 5-HT(2A) receptor mRNA levels in the CA3 subregion. These results suggest that impaired glucocorticoid receptor function can affect hippocampal 5-HT(2A) receptor expression in transgenic mice and that this is not corrected by fluoxetine treatment. Copyright 2001 S. Karger AG, Basel |
