| First Author | Iakoubova OA | Year | 2000 |
| Journal | Physiol Genomics | Volume | 3 |
| Issue | 3 | Pages | 145-8 |
| PubMed ID | 11015609 | Mgi Jnum | J:65448 |
| Mgi Id | MGI:1926588 | Doi | 10.1152/physiolgenomics.2000.3.3.145 |
| Citation | Iakoubova OA, et al. (2000) Microsatellite marker panels for use in high-throughput genotyping of mouse crosses. Physiol Genomics 3(3):145-8 |
| abstractText | Several microsatellite genotyping panel sets have been developed that are polymorphic between C57BL/6J and CAST/Ei mice, or C57BL/6J and DBA/2J. One set of markers for each strain pair has an intermarker distance of approximately 20 cM, and a second set has an intermarker distance of 5 cM. The 20-cM set contains 105 markers for C57BL/6J x DBA/2J and 108 for C57BL/6J x CAST/Ei, divided into 13 panels. Each 5-cM set includes 350 markers arranged into 45 panels. A panel contains a number of primer pairs whose fluorescently labeled PCR products can be pooled together and separated on one lane of a polyacrylamide gel. The sets are arranged by the size of the PCR product and by the type of fluorescent dye; 5-cM sets are also arranged by chromosomal region. The 20-cM sets are most useful for full-genome scans, the 5-cM sets are useful for full-genome and/or for region-specific chromosome screens. Both sets were proven as useful tools for speed congenic development, quantitative trait loci (QTL) analysis and physical mapping. These panel sets provide a throughput of 1,536-2,304 mouse genotypes daily per one gel-based system. Whole genome scans of one animal require 13 or 48 gel lanes, with 20 cM or 5 cM density, respectively. |
