| First Author | Takahashi T | Year | 2001 |
| Journal | Biochem Biophys Res Commun | Volume | 281 |
| Issue | 4 | Pages | 1057-62 |
| PubMed ID | 11237772 | Mgi Jnum | J:68000 |
| Mgi Id | MGI:1931913 | Doi | 10.1006/bbrc.2001.4463 |
| Citation | Takahashi T, et al. (2001) Cloning of Hypoxia-Inducible Factor 1alpha cDNA from Chick Embryonic Ventricular Myocytes. Biochem Biophys Res Commun 281(4):1057-62 |
| abstractText | Hypoxia-inducible factor 1 (HIF-1) is composed of HIF-1alpha and arylhydocarbon nuclear receptor translocator (ARNT), which belong to the basic-helix-loop-helix-Per/ARNT/Sim (bHLH-PAS) family of transcription factors. HIF plays key roles in oxygen homeostasis and embryonic cardiovascular development. In this study, we have cloned cDNAs encoding the chick HIF-1alpha from cultured embryonic ventricular myocytes (CEVM) and then examined its expression in various embryonic tissues. The deduced amino acid sequence of the chick HIF-1alpha cDNA showed 79% identity with that of the human HIF-1alpha cDNA. In contrast, sequence homology between the chick HIF-1alpha and endothelial PAS protein 1 (EPAS1), another member of the bHLH-PAS proteins, was only low (49%). HIF-1alpha mRNA was expressed abundantly in CEVM, but scarcely in the liver, which was quite different from expression pattern of EPAS1 mRNA. These data suggest that HIF-1alpha may be involved in embryonic cardiovascular development. HIF-1alpha and EPAS1 may play distinct roles during developmental stages. Copyright 2001 Academic Press. |
