| First Author | Dong H | Year | 2001 |
| Journal | Gene | Volume | 264 |
| Issue | 2 | Pages | 187-96 |
| PubMed ID | 11250073 | Mgi Jnum | J:68184 |
| Mgi Id | MGI:1932231 | Doi | 10.1016/s0378-1119(01)00335-3 |
| Citation | Dong H, et al. (2001) Genomic sequence and expression analyses of human chromatin assembly factor 1 p150 gene. Gene 264(2):187-96 |
| abstractText | Chromatin assembly factor-1 (CAF-1) plays essential roles in eukaryotic chromatin assembly during DNA replication (Smith and Stillman, 1989. Cell 58, 15-25), (Krude, 1999. Eur. J. Biochem. 263, 1-5). Its p150 subunit, involved in interaction with histone H3 and H4, is critical to the CAF-1 nucleosome assembly activity. In this study, we sequenced a 96-kb genomic DNA region that includes a 42.8-kb CAF-1 p150 subunit gene (CHAF1A), and a 41.1-kb EEN gene. A scripted bioinformatics analysis pipeline (research agent) has been set up to annotate the BAC sequence with a set of integrated algorithms. The CAF-1 p150 subunit gene contains 15 exons and 14 introns. The promoter region is characterized by deletional analyses, revealing a potential repressor. Tissue-correlated alternative splicing forms of the transcript was initially identified by EST clustering analysis, then confirmed by RT-PCR which resulted more splicing forms than computational prediction. |
