| First Author | Yamawaki K | Year | 2001 |
| Journal | Biochem Biophys Res Commun | Volume | 285 |
| Issue | 4 | Pages | 1040-5 |
| PubMed ID | 11467857 | Mgi Jnum | J:70734 |
| Mgi Id | MGI:2138054 | Doi | 10.1006/bbrc.2001.5290 |
| Citation | Yamawaki K, et al. (2001) Identification of Human CPI-17, an Inhibitory Phosphoprotein for Myosin Phosphatase. Biochem Biophys Res Commun 285(4):1040-5 |
| abstractText | CPI-17 is a phosphorylation-dependent inhibitor of myosin phosphatase. cDNA clones encoding CPI-17 were isolated from a human aorta library. Overlapping clones indicated two isoforms: CPI-17alpha was 147 residues and mass of 16.7 kDa; CPI-17beta (120 residues, mass 13.5 kDa) resulted from a deletion in the alpha-isoform of 27 residues, sequence 68-94. N-terminal 67 residues of all CPI-17 isoforms (human, porcine, rat and mouse) were highly conserved (for the human and porcine isoforms the identity was 91%). The presence of the two human isoforms was detected from cDNA sequences amplified by RT-PCR and by Western blots on human aorta. The cloned human CPI-17 gene indicated 4 coding exons and CPI-17beta was an alternative splice variant due to deletion of the second exon. FISH analysis located the human CPI-17 gene on chromosome 19q13.1. Copyright 2001 Academic Press. |
