| First Author | Eubank DW | Year | 2001 |
| Journal | Biochem Biophys Res Commun | Volume | 285 |
| Issue | 3 | Pages | 811-9 |
| PubMed ID | 11453665 | Mgi Jnum | J:70663 |
| Mgi Id | MGI:2137973 | Doi | 10.1006/bbrc.2001.5236 |
| Citation | Eubank DW, et al. (2001) C/EBPbeta interacts with the P-enolpyruvate carboxykinase adipocyte-specific enhancer. Biochem Biophys Res Commun 285(3):811-9 |
| abstractText | CCAAT/enhancer binding protein (C/EBP) family members are known to transactivate the gene encoding cytosolic phosphoenolpyruvate carboxykinase (PEPCK; EC 4.1.1.32) in hepatocytes via promoter proximal C/EBP response elements. PEPCK is also expressed in adipocytes; however, fibroblasts that are homozygous null for C/EBPbeta cannot express PEPCK when induced to differentiate into adipocytes (Tanaka et al., EMBO J. 16, 7432-7443, 1997). This along with our previous observation that an upstream adipocyte-specific enhancer contains multiple putative C/EBP binding elements suggested the possibility that C/EBPbeta transactivates the PEPCK gene in adipocytes via distal elements. We report here that C/EBPbeta transactivates a PEPCK-luciferase chimera in transient transfection assays. C/EBPbeta acted independently of peroxisome proliferator-activated receptor gamma (PPARgamma) which is required for function of the enhancer. C/EBPbeta in nuclear extracts and recombinant C/EBPbeta bound three of the putative C/EBP-binding elements within the enhancer. C/EBPbeta binding to these three elements was strongly cooperative. However, mutation of all three elements did not affect reporter transactivation by C/EBPbeta suggesting that additional elements participate in PEPCK regulation or that the effects of C/EBPbeta are indirect. Copyright 2001 Academic Press. |
