| First Author | Kikuchi K | Year | 2001 |
| Journal | Int Immunol | Volume | 13 |
| Issue | 6 | Pages | 777-83 |
| PubMed ID | 11369705 | Mgi Jnum | J:69777 |
| Mgi Id | MGI:2135428 | Doi | 10.1093/intimm/13.6.777 |
| Citation | Kikuchi K, et al. (2001) Suppression of thymic development by the dominant-negative form of Gads. Int Immunol 13(6):777-83 |
| abstractText | Gads, a hematopoietic-lineage-specific Grb2 family member, is involved in the signaling mediated by the TCR through its interactions with SLP-76 and LAT. Here, we generated transgenic mice expressing Grf40-dSH2, an SH2-deleted dominant-negative form of Gads, which is driven by the lck proximal promoter. The total number of thymocytes was profoundly reduced in the transgenic mice, whereas in the double-negative (CD4(-)CD8(-)) thymocyte subset, in particular the CD25(+)CD44(-) pre-T cell population, it was significantly increased. However, CD5 expression, which is mediated by pre-TCR stimulation, was significantly suppressed on the CD4(-)CD8(-) thymocytes of the transgenic mice. Furthermore, the SLP-76-dependent signaling was markedly suppressed as well. These data suggest that Gads plays an important role in the pre-TCR as well as TCR signaling in thymocytes. |
