| First Author | Ward H | Year | 2001 |
| Journal | Cytokine | Volume | 15 |
| Issue | 5 | Pages | 237-40 |
| PubMed ID | 11594787 | Mgi Jnum | J:71991 |
| Mgi Id | MGI:2151344 | Doi | 10.1006/cyto.2001.0933 |
| Citation | Ward H, et al. (2001) THE RAT INTERLEUKIN 10 RECEPTOR: CLONING AND SEQUENCING OF cDNA CODING FOR THE ALPHA-CHAIN PROTEIN SEQUENCE, AND DEMONSTRATION BY WESTERN BLOTTING OF EXPRESSION IN THE RAT BRAIN. Cytokine 15(5):237-40 |
| abstractText | cDNA coding for the alpha chain of the rat interleukin 10 (IL-10) receptor was amplified by polymerase chain reaction (PCR), cloned and sequenced. The nucleic acid coding sequence exhibited 88% and 68% homology with the mouse and human IL-10 receptor sequences, respectively. The translated protein exhibited 83% and 61% homology with the mouse and human IL-10 receptor proteins. Specific antibodies were raised to the extracellular domain of the rat IL-10 receptor expressed as a secreted protein in recombinant Drosophila S2 cells. Western blotting using these antibodies demonstrated the presence of the IL-10 receptor in five major regions of the rat brain (cortex, cerebellum, hippocampus, hypothalamus and pituitary), supporting a role for IL-10 as a central regulator of inflammation. |
