| First Author | Li Y | Year | 2001 |
| Journal | J Biol Chem | Volume | 276 |
| Issue | 44 | Pages | 40824-33 |
| PubMed ID | 11524423 | Mgi Jnum | J:72535 |
| Mgi Id | MGI:2153221 | Doi | 10.1074/jbc.M106141200 |
| Citation | Li Y, et al. (2001) Spring, a Novel RING Finger Protein That Regulates Synaptic Vesicle Exocytosis. J Biol Chem 276(44):40824-33 |
| abstractText | The synaptosome-associated protein of 25 kDa (SNAP-25) interacts with syntaxin 1 and vesicle-associated membrane protein 2 (VAMP2) to form a ternary soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptor (SNARE) complex that is essential for synaptic vesicle exocytosis. We report a novel RING finger protein, Spring, that specifically interacts with SNAP-25. Spring is exclusively expressed in brain and is concentrated at synapses. The association of Spring with SNAP-25 abolishes the ability of SNAP-25 to interact with syntaxin 1 and VAMP2 and prevents the assembly of the SNARE complex. Overexpression of Spring or its SNAP-25-interacting domain reduces Ca(2+)-dependent exocytosis from PC12 cells. These results indicate that Spring may act as a regulator of synaptic vesicle exocytosis by controlling the availability of SNAP-25 for the SNARE complex formation. |
