| First Author | Thoelen I | Year | 2001 |
| Journal | Biochem Biophys Res Commun | Volume | 288 |
| Issue | 4 | Pages | 805-8 |
| PubMed ID | 11688979 | Mgi Jnum | J:72612 |
| Mgi Id | MGI:2153306 | Doi | 10.1006/bbrc.2001.5851 |
| Citation | Thoelen I, et al. (2001) Characterization of a cDNA encoding the bovine coxsackie and adenovirus receptor. Biochem Biophys Res Commun 288(4):805-8 |
| abstractText | Non-human adenoviruses such as bovine adenovirus type 3 (BAV-3) that do not replicate in human cells but can infect human cells in culture could provide an attractive alternative to human adenoviral vectors for gene therapy. In addition, a large-animal model for genetic diseases can be very useful for the assessment of the efficacy of adenovector-mediated gene delivery in man. Recombinant human subgroup C adenovectors use the coxsackie and adenovirus receptor (CAR) to enter their target cells. Through RT-PCR and sequencing we determined the complete coding sequence of bovine CAR which serves as the primary adenoviral attachment site on bovine cells. A multiple sequence alignment, involving all the previously identified CAR species (man, mouse, rat, pig, and dog) showed that bovine CAR was most related to porcine CAR (92% nucleotide similarity) and demonstrated a highly conserved adenovirus binding Ig1 domain. Copyright 2001 Academic Press. |
