| First Author | Rainio EM | Year | 2002 |
| Journal | J Immunol | Volume | 168 |
| Issue | 4 | Pages | 1524-7 |
| PubMed ID | 11823475 | Mgi Jnum | J:74465 |
| Mgi Id | MGI:2158519 | Doi | 10.4049/jimmunol.168.4.1524 |
| Citation | Rainio EM, et al. (2002) Cutting Edge: Transcriptional Activity of NFATc1 Is Enhanced by the Pim-1 Kinase. J Immunol 168(4):1524-7 |
| abstractText | Pim-1 is an oncogenic serine/threonine kinase implicated in cytokine-induced signal transduction and in development of lymphoid malignancies. However, its precise function as well as physiological substrates have remained unknown. In this study we demonstrate that Pim-1 can physically interact with the NFATc1 transcription factor and phosphorylate it in vitro on several serine residues. In contrast to previously recognized NFATc kinases, wild-type Pim-1 enhances NFATc-dependent transactivation and IL-2 production in Jurkat T cells, while kinase-deficient Pim-1 mutants inhibit them in a dominant negative fashion. Our results reveal a novel, phosphorylation-dependent regulatory mechanism targeting NFATc1 through which Pim-1 acts as a downstream effector of Ras to facilitate IL-2-dependent proliferation and/or survival of lymphoid cells. |
