| First Author | Noma T | Year | 1999 |
| Journal | Biochem Biophys Res Commun | Volume | 264 |
| Issue | 3 | Pages | 990-7 |
| PubMed ID | 10544043 | Mgi Jnum | J:58396 |
| Mgi Id | MGI:1347442 | Doi | 10.1006/bbrc.1999.1616 |
| Citation | Noma T, et al. (1999) Cloning and functional characterization of the promoter region of the gene encoding human adenylate kinase isozyme 3. Biochem Biophys Res Commun 264(3):990-7 |
| abstractText | The 5'-flanking region of the gene encoding human adenylate kinase isozyme 3 was isolated and compared with that of the bovine AK3 gene previously characterized. Four conserved DNA sequences (elements-a, -b, -c, and -d) were found in both the regions. The promoter activities were analyzed in HeLa cells using promoter-CAT reporter constructs. The proximal promoter region (-217 to +261), which contains three of four conserved elements, gave a maximum promoter activity. In a series of electrophoretic mobility-shift assays, DNA fragments and double-stranded oligodeoxyribonucleotides containing sequences of the four conserved elements interacted with nuclear extracts of HeLa cells. The a-element contained the W-element, while the d-element, which had a high G + C content, was a novel regulatory cis-element distinct from the GC box. The b- and c-elements were homologous to each other and had a motif resembling downstream promoter element. Mutations of the c- and d-elements significantly reduced the promoter activity, indicating that the c- and d-elements in the AK3 promoter are crucial. These elements may also be involved in the transcriptional regulation of other TATA-less genes. Copyright 1999 Academic Press. |
