| First Author | Toyoda-Ohno H | Year | 1999 |
| Journal | Dev Biol | Volume | 215 |
| Issue | 2 | Pages | 399-406 |
| PubMed ID | 10545246 | Mgi Jnum | J:58414 |
| Mgi Id | MGI:1347642 | Doi | 10.1006/dbio.1999.9482 |
| Citation | Toyoda-Ohno H, et al. (1999) Members of the ErbB receptor tyrosine kinases are involved in germ cell development in fetal mouse gonads. Dev Biol 215(2):399-406 |
| abstractText | To isolate the genes involved in mouse primordial germ cell (PGC) development, we carried out subtraction cDNA cloning between PGC-derived embryonic germ (EG) cells and inner cell mass-derived embryonic stem cells. Among the genes preferentially expressed in EG cells, we found a gene encoding a receptor tyrosine kinase ErbB3. By in situ hybridization and immunohistochemical staining, the expression of ErbB3 as well as that of ErbB2, a coreceptor for ErbB3, was detected in PGCs in genital ridges at 12.5 dpc (days postcoitum). The expression was, however, downregulated at 14.5 dpc when the PGCs underwent growth cessation. Neuregulin-beta, a ligand for ErbB2 and ErbB3, was also expressed in genital ridges. In addition, a recombinant Neuregulin-beta enhanced the number of PGCs in 12.5-dpc embryos in culture. Taken together, these observations suggest that ErbB signaling controls the growth or survival of PGCs in genital ridges. Copyright 1999 Academic Press. |
