| First Author | Yan Z | Year | 2000 |
| Journal | Mol Cell Biol | Volume | 20 |
| Issue | 3 | Pages | 1021-9 |
| PubMed ID | 10629059 | Mgi Jnum | J:59996 |
| Mgi Id | MGI:1352378 | Doi | 10.1128/mcb.20.3.1021-1029.2000 |
| Citation | Yan Z, et al. (2000) PR48, a novel regulatory subunit of protein phosphatase 2A, interacts with Cdc6 and modulates DNA replication in human cells. Mol Cell Biol 20(3):1021-9 |
| abstractText | Initiation of DNA replication in eukaryotes is dependent on the activity of protein phosphatase 2A (PP2A), but specific phosphoprotein substrates pertinent to this requirement have not been identified. A novel regulatory subunit of PP2A, termed PR48, was identified by a yeast two-hybrid screen of a human placental cDNA library, using human Cdc6, an essential component of prereplicative complexes, as bait. PR48 binds specifically to an amino-terminal segment of Cdc6 and forms functional holoenzyme complexes with A and C subunits of PP2A. PR48 localizes to the nucleus of mammalian cells, and its forced overexpression perturbs cell cycle progression, causing a G(1) arrest. These results suggest that dephosphorylation of Cdc6 by PP2A, mediated by a specific interaction with PR48, is a regulatory event controlling initiation of DNA replication in mammalian cells. |
