| First Author | Morafo V | Year | 1999 |
| Journal | Cell Immunol | Volume | 198 |
| Issue | 2 | Pages | 77-86 |
| PubMed ID | 10648121 | Mgi Jnum | J:60260 |
| Mgi Id | MGI:1353101 | Doi | 10.1006/cimm.1999.1584 |
| Citation | Morafo V, et al. (1999) Induction of a germinal center phenotype in B cells in vitro by a Th2 cell line. Cell Immunol 198(2):77-86 |
| abstractText | We have investigated the contribution of various stimuli for generating in vitro the changes in surface phenotype characteristic of B cells responding to a T-dependent antigen in a germinal center (GC). We show that, unlike many other stimuli such as B cell mitogens, cytokines, and surrogate antigen, alone or in combination, an alloreactive Th2 clonal line induces splenic B cells to become cell surface peanut agglutinin (PNA)(hi), Ig(lo), CD62L(lo), and CD44(hi) to produce mRNA for M17 and to express a GC-specific transgene even without B cell receptor ligation. Neither proliferation nor prior activation of responding B cells is needed, but B cells from CD45-null mice show reduced efficiency of this induction. These findings open up possibilities for separation and dissection of the various components of the GC response. Copyright 1999 Academic Press. |
