| First Author | Konrad L | Year | 1999 |
| Journal | Biochim Biophys Acta | Volume | 1489 |
| Issue | 2-3 | Pages | 440-4 |
| PubMed ID | 10673048 | Mgi Jnum | J:59136 |
| Mgi Id | MGI:1351071 | Doi | 10.1016/s0167-4781(99)00208-0 |
| Citation | Konrad L, et al. (1999) Calcyclin is differentially expressed in rat testicular cells. Biochim Biophys Acta 1489(2-3):440-4 |
| abstractText | Immature rat Sertoli cells aggregate and form tubule-like structures when cultured on a monolayer of peritubular myoid cells. In this study, differential gene expression of monocultures and direct cocultures of peritubular cells and Sertoli cells were examined. One of the cDNA clones isolated showed high homology to calcyclin and a microvascular differentiation gene, CEC5, which was reported to be highly homologous to CASK, a membrane-associated guanylate kinase homolog. Sequencing and mRNA analysis of rat calcyclin demonstrated that the gene was differentially expressed and was found only in peritubular cells and cocultures with increased levels. In contrast, CASK was expressed by Sertoli cells, peritubular cells, and cocultures, whereas CEC5 was never found in the testicular somatic cells. Our findings point to a paracrine regulation of calcyclin expression in testicular peritubular fibroblasts which seems to be related to tubular growth. |
