| First Author | Hallensleben W | Year | 2000 |
| Journal | J Immunol Methods | Volume | 234 |
| Issue | 1-2 | Pages | 149-51 |
| PubMed ID | 10669779 | Mgi Jnum | J:60300 |
| Mgi Id | MGI:1353143 | Doi | 10.1016/s0022-1759(99)00197-0 |
| Citation | Hallensleben W, et al. (2000) A polymorphism in the mouse crg-2/IP-10 gene complicates chemokine gene expression analysis using a commercial ribonuclease protection assay. J Immunol Methods 234(1-2):149-51 |
| abstractText | The use of multiprobe RPAs is becoming an increasingly popular method for the detection and quantitation of RNA levels in cells and tissues. Here we report that due to a polymorphism in the 3'-noncoding region of the mouse Crg-2/IP-10 gene, the mCK-5 chemokine probe set available from Pharmingen can yield aberrant signal patterns with RNA samples from BALB/c, MRL and possibly other mouse strains that may lead to false conclusions regarding expression of the Crg-2/IP-10 and MCP-1 genes. |
