| First Author | Pietschmann SM | Year | 2000 |
| Journal | Am J Physiol Lung Cell Mol Physiol | Volume | 278 |
| Issue | 4 | Pages | L765-78 |
| PubMed ID | 10749754 | Mgi Jnum | J:61594 |
| Mgi Id | MGI:1355200 | Doi | 10.1152/ajplung.2000.278.4.L765 |
| Citation | Pietschmann SM, et al. (2000) cDNA cloning of ovine pulmonary SP-A, SP-B, and SP-C: isolation of two different sequences for SP-B. Am J Physiol Lung Cell Mol Physiol 278(4):L765-78 |
| abstractText | Pulmonary surfactant promotes alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. The three surfactant proteins SP-A, SP-B, and SP-C contribute to dynamic surface properties involved during respiration. We have cloned and sequenced the complete cDNAs for ovine SP-A and SP-C and two distinct forms of ovine SP-B cDNAs. The nucleotide sequence of ovine SP-A cDNA consists of 1,901 bp and encodes a protein of 248 amino acids. Ovine SP-C cDNA contains 809 bp, predicting a protein of 190 amino acids. Ovine SP-B is encoded by two mRNA species, which differ by a 69-bp in-frame deletion in the region coding for the active airway protein. The larger SP-B cDNA comprises 1,660 bp, encoding a putative protein of 374 amino acids. With the sequences reported, a more complete analysis of surfactant regulation and the determination of their physiological function in vivo will be enabled. |
