| First Author | Belen Carrillo M | Year | 2000 |
| Journal | Infect Immun | Volume | 68 |
| Issue | 5 | Pages | 2728-34 |
| PubMed ID | 10768966 | Mgi Jnum | J:61695 |
| Mgi Id | MGI:1355434 | Doi | 10.1128/iai.68.5.2728-2734.2000 |
| Citation | Belen Carrillo M, et al. (2000) Heterologous expression of trypanosoma cruzi trans-sialidase in leishmania major enhances virulence. Infect Immun 68(5):2728-34 |
| abstractText | Earlier studies showed that mice primed for a few hours with the trans-sialidase (TS) of Trypanosoma cruzi, the agent of Chagas' disease, become highly susceptible to trypanosomal infection. These studies suggest that TS affects parasite virulence independent of antigenic stimulation. Potentially, TS could enhance or reduce the virulence of heterologous microbes depending on the mechanism of TS action and on the type of immune response elicited by the particular parasite. We tested this hypothesis by expressing heterologous TS in Leishmania major, a protozoan parasite that causes cutaneous leishmaniasis and lacks TS and the TS product alpha2-3-linked sialic acid. Leishmania cells transfected with a T. cruzi TS expression construct made high levels of active enzyme, which was present in the promastigotes and shed into the extracellular milieu. TS expression did not affect L. major binding to and entry into cultured macrophages or its tropism for macrophage infection in vivo. However, TS-expressing L. major exhibited elevated virulence in BALB/c mice, as determined by lesion progression, parasite numbers, and macro- and microscopic examination of cutaneous lesions. Several genetic tests proved that the enhanced virulence was directly attributable to TS expression. The results are consistent with TS functioning to sabotage the mouse immune system to confer a growth advantage on T. cruzi and transgenic L. major. These data suggest that heterologous expression of T. cruzi virulence factors in Leishmania may provide a new approach for dissecting their function in vivo. |
