| First Author | Pierce M | Year | 2000 |
| Journal | Biochim Biophys Acta | Volume | 1490 |
| Issue | 1-2 | Pages | 175-83 |
| PubMed ID | 10786634 | Mgi Jnum | J:60267 |
| Mgi Id | MGI:1353108 | Doi | 10.1016/s0167-4781(99)00229-8 |
| Citation | Pierce M, et al. (2000) Nucleotide sequence of a cDNA clone coding for an intestinal-type fatty acid binding protein and its tissue-specific expression in zebrafish (Danio rerio). Biochim Biophys Acta 1490(1-2):175-83 |
| abstractText | We have cloned a cDNA from zebrafish (Danio rerio) that contains an open-reading frame of 132 amino acids coding for a fatty acid binding protein (FABP) of approximately 15 kDa. Multiple sequence alignment revealed extensive amino acid identity between this zebrafish FABP and intestinal-like FABPs (I-FABP) from other species. The zebrafish I-FABP cDNA hybridized to single restriction fragments of total zebrafish genomic DNA digested with the restriction endonucleases PstI Bg/II or EcoRI suggesting that a single copy of the I-FABP gene is present in the zebrafish genome. An oligonucleotide probe complementary to the zebrafish I-FABP mRNA hybridized to an mRNA of approximately 800 bases in Northern blot analysis. In situ hybridization revealed that the I-FABP mRNA was expressed exclusively in the intestine of the adult zebrafish. |
