| First Author | Edashige K | Year | 2000 |
| Journal | Cryobiology | Volume | 40 |
| Issue | 2 | Pages | 171-5 |
| PubMed ID | 10788316 | Mgi Jnum | J:63371 |
| Mgi Id | MGI:1860820 | Doi | 10.1006/cryo.1999.2228 |
| Citation | Edashige K, et al. (2000) Expression of mRNAs of the aquaporin family in mouse oocytes and embryos. Cryobiology 40(2):171-5 |
| abstractText | The molecular basis of water and cryoprotectant permeability in mammalian oocytes and embryos is poorly understood. Therefore, we investigated the expression of mRNAs of water channel proteins (aquaporins) in mouse oocytes and embryos by RT-PCR. The total RNA of mouse oocytes at metaphase II and embryos at the 4-cell, morula, and blastocyst stages was isolated, reverse-transcribed, and subjected to nested PCR amplification. Aquaporins were expressed in both oocytes and embryos, but the types were different among the developmental stages: aquaporins 3 and 7 were expressed in oocytes and embryos at all stages examined, but aquaporins 8 and 9 were expressed only in blastocysts. On the other hand, aquaporins 1, 2, 4, 5, and 6 were not detected in any of the stages examined. The present study shows for the first time that aquaporins are expressed in mammalian oocytes and embryos. These aquaporins may play a role in water transport and conceivably also in cryoprotectant transport across the plasma membrane in these cells. Copyright 2000 Academic Press. |
