| First Author | McKenzie AW | Year | 2000 |
| Journal | Transplantation | Volume | 69 |
| Issue | 8 | Pages | 1745-8 |
| PubMed ID | 10836396 | Mgi Jnum | J:61907 |
| Mgi Id | MGI:1855756 | Doi | 10.1097/00007890-200004270-00041 |
| Citation | McKenzie AW, et al. (2000) Local secretion of a chimeric anti-CD4 antibody protects against graft rejection in the NOD mouse. Transplantation 69(8):1745-8 |
| abstractText | BACKGROUND: Engineering a graft to secrete its own immunosuppressive antibodies may minimize the risks associated with current high dose systemic immunosuppression. METHODS AND RESULTS: A beta cell insulinoma cell line (NIT-1) was transfected with genes encoding a chimeric anti-CD4 antibody. The NIT-1 cells secreted functional chimeric anti-CD4 antibody that bound to the CD4 molecule on mouse thymocytes and inhibited in vitro proliferation of CD4+ve T cells. Both test and control transfected cell lines grew at a similar rate in immunodeficient mice. In immunocompetent NOD mice, NIT-1 cells are normally rejected by a cellular immune response against the SV40 T antigen. Although control transfected NIT-1 cells were rapidly rejected by NOD mice, anti-CD4 secreting NIT-1 cells grew significantly better and were able to form tumors at the site of injection. CONCLUSIONS: The local secretion of chimeric anti-CD4 antibody from transfected cells can contribute to graft survival in our transplantation model. |
