| First Author | Kuriyama H | Year | 2000 |
| Journal | Gene | Volume | 253 |
| Issue | 2 | Pages | 151-60 |
| PubMed ID | 10940552 | Mgi Jnum | J:63939 |
| Mgi Id | MGI:1888435 | Doi | 10.1016/s0378-1119(00)00247-x |
| Citation | Kuriyama H, et al. (2000) Characterization and chromosomal mapping of a novel human gene, ANKHZN. Gene 253(2):151-60 |
| abstractText | Ankhzn (ankyrin repeats hooked to a zinc finger motif) was originally isolated by means of the gene trap method, as a novel cytoplasmic protein on mouse embryonic stem cells. The Ankhzn protein is ubiquitously expressed in a spatiotemporal-specific manner and is located on endosomes. In the present study, we have cloned human ANKHZN cDNA by PCR using candidate EST clones exhibiting a high homology to mouse Ankhzn cDNA. The human ANKHZN cDNA encoded a 1166aa protein exhibiting 84.9% identity to the mouse one. The size of the transcript was found to be about 7kb on a Northern blot analysis, and ANKHZN mRNA was found to be ubiquitously expressed in human tissues on RT-PCR analysis. Western blot analysis showed that a 130kDa protein was detected at various levels in human tissues and also present in both membrane and soluble fractions obtained on subcellular fractionation. Human ANKHZN is a single copy gene consisting of predicted 25 exons in the human genome, and has been mapped to human chromosome 17p13 by radiation hybrid panel and fluorescence in-situ hybridization. |
