| First Author | Yang S | Year | 2000 |
| Journal | J Interferon Cytokine Res | Volume | 20 |
| Issue | 9 | Pages | 779-85 |
| PubMed ID | 11032397 | Mgi Jnum | J:65340 |
| Mgi Id | MGI:1926392 | Doi | 10.1089/10799900050151049 |
| Citation | Yang S, et al. (2000) Canine interleukin-13: molecular cloning of full-length cDNA and expression of biologically active recombinant protein. J Interferon Cytokine Res 20(9):779-85 |
| abstractText | Interleukin-13 (IL-13) regulates immune responses mediated by type 2 T helper lymphocytes (Th2) in the human and mouse. To study the function of this cytokine in the dog, we have isolated a cDNA that encodes the full-length canine IL-13 (CaIL-13) precursor polypeptide of 131 amino acids. CaIL-13 shares significant homology with the IL-13 amino acid sequences of cattle (54.1%), mouse (39.6%), and rat (36.6%) but shares the highest identity with human IL-13 (HuIL-13) (61.8%). The predicted CaIL-13 mature polypeptide of 111 residues was expressed in bacteria, and recombinant CaIL-13 (rCaIL-13) was isolated from inclusion bodies and refolded. rCaIL-13 stimulated the proliferation of TF-1 cells, which are derived from human erythroleukemia cells and respond to IL-13 as well as to a number of other human and murine cytokines. CaIL-13 mRNA was readily detectable by reverse transcriptase-polymerase chain reaction (RT-PCR) in cells from lymph nodes and peripheral blood. The gene sequence and biologically active recombinant protein for CaIL-13 will be useful reagents to determine the role of IL-13 in the regulation of canine immune responses. |
