| First Author | Scully KM | Year | 2000 |
| Journal | Science | Volume | 290 |
| Issue | 5494 | Pages | 1127-31 |
| PubMed ID | 11073444 | Mgi Jnum | J:65820 |
| Mgi Id | MGI:1927333 | Doi | 10.1126/science.290.5494.1127 |
| Citation | Scully KM, et al. (2000) Allosteric effects of pit-1 DNA sites on long-term repression in cell type specification. Science 290(5494):1127-31 |
| abstractText | Reciprocal gene activation and restriction during cell type differentiation from a common lineage is a hallmark of mammalian organogenesis. A key question, then, is whether a critical transcriptional activator of cell type-specific gene targets can also restrict expression of the same genes in other cell types. Here, we show that whereas the pituitary-specific POU domain factor Pit-1 activates growth hormone gene expression in one cell type, the somatotrope, it restricts its expression from a second cell type, the lactotrope. This distinction depends on a two-base pair spacing in accommodation of the bipartite POU domains on a conserved growth hormone promoter site. The allosteric effect on Pit-1, in combination with other DNA binding factors, results in the recruitment of a corepressor complex, including nuclear receptor corepressor N-CoR, which, unexpectedly, is required for active long-term repression of the growth hormone gene in lactotropes. |
