| First Author | Abdallah MA | Year | 2000 |
| Journal | Biochem Genet | Volume | 38 |
| Issue | 5-6 | Pages | 157-65 |
| PubMed ID | 11091906 | Mgi Jnum | J:65586 |
| Mgi Id | MGI:1926764 | Citation | Abdallah MA, et al. (2000) Identification and characterization of a cDNA clone encoding the heat shock protein (Hsp60) from the biting midge, Culicoides variipennis sonorensis Wirth and Jones. Biochem Genet 38(5-6):157-65 |
| abstractText | A cDNA expression library constructed from Culicoides variipennis sonorensis was screened using an antibody specific for Hsp60 of Heliothis virescens. A single clone encoding the complete heat shock protein (Hsp60) of C. variipennis was identified and its 2400-bp insert was sequenced. The encoded 62-kDa protein contains 581 amino acids and includes a 26-amino acid putative mitochondrial targeting sequence at its N terminus and a GGM motif at its carboxyl terminus. Deduced amino acid sequences are highly similar (67-78%) to Hsp60 of other species, including the fruit fly, the house mouse, the Norwegian rat, the Chinese hamster, the human, a nematode, and the tobacco budworm moth. This is the initial isolation of a coding sequence for a stress-induced protein in C. variipennis. |
