| First Author | Brack C | Year | 1977 |
| Journal | Proc Natl Acad Sci U S A | Volume | 74 |
| Issue | 12 | Pages | 5652-6 |
| PubMed ID | 414223 | Mgi Jnum | J:32611 |
| Mgi Id | MGI:80105 | Doi | 10.1073/pnas.74.12.5652 |
| Citation | Brack C, et al. (1977) Variable and constant parts of the immunoglobulin light chain gene of a mouse myeloma cell are 1250 nontranslated bases apart. Proc Natl Acad Sci U S A 74(12):5652-6 |
| abstractText | A DNA fragment carrying an immunoglobulin gene coding for both variable (V) and constant (C) regions of a mouse lambda light chain was enriched about 15-fold from a total endonuclease EcoRI digest of a plasmacytoma (HOPC 2020) DNA by preparative agarose gel electrophoresis. The DNA fraction was used for cloning of a lambda chain gene in the phage lambdagtWES vector. After screening [Benton, W. D. & Davis, R. W. (1977) Science 196, 180-182] of about 70,000 plaques, each arising from an independent transfection event, we isolated one clone (Ig 303) that contained both a Vlambda and a Clambda DNA sequence. Electron microscopy of R-loops formed between the cloned DNA and purified lambda chain mRNA (HOPC 2020) revealed that the Vlambda and Clambda DNA sequences are separated by a 1250-base DNA fragment. |
