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Publication : Construction of recombinant plasmids containing rat muscle actin and myosin light chain DNA sequences.

First Author  Katcoff D Year  1980
Journal  Proc Natl Acad Sci U S A Volume  77
Issue  2 Pages  960-4
PubMed ID  6928693 Mgi Jnum  J:6283
Mgi Id  MGI:54760 Doi  10.1073/pnas.77.2.960
Citation  Katcoff D, et al. (1980) Construction of recombinant plasmids containing rat muscle actin and myosin light chain DNA sequences. Proc Natl Acad Sci U S A 77(2):960-4
abstractText  The construction and partial characterization of recombinant bacterial plasmids carrying DNA sequences that hybridize with rat skeletal muscle actin and a myosin light chain mRNA is described. DNA of one clone hybridizes specifically with the muscle-specific alpha-actin mRNA. Three plasmid clones contain DNA inserts that hybridize with muscle as well as with nonmuscle actin mRNA. A fifth plasmid contains sequences complementary to mRNA coding for myosin light chain 2. DNA of this plasmid hybridizes specifically with RNA extracted from muscle and differentiated muscle cultures but not with RNA extracted from proliferating mononucleated myogenic cells.
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