| First Author | Marcu KB | Year | 1980 |
| Journal | Cell | Volume | 22 |
| Issue | 1 Pt 1 | Pages | 187-96 |
| PubMed ID | 6775817 | Mgi Jnum | J:6394 |
| Mgi Id | MGI:54870 | Doi | 10.1016/0092-8674(80)90167-1 |
| Citation | Marcu KB, et al. (1980) 5' flanking region of immunoglobulin heavy chain constant region genes displays length heterogeneity in germlines of inbred mouse strains. Cell 22(1 Pt 1):187-96 |
| abstractText | Genomic Southern blotting analysis reveals that a 5' flanking DNA sequence located 1.5-2.0 kb upstream from the C mu and C alpha genes undergoes extensive length variation in the germlines of inbred and wild mice. In addition, genomic clones of the BALB/c C mu gene undergo deletion events during their propagation in Charon 4A when maintained in RecA+ bacterial strains. These deletion events occur between the JH cluster and the C mu gene and approximately 2.0 kb 5' of the C alpha gene, and may encompass several kb of DNA. Deletion events are capable of yielding a group of C mu clones which differ in size by units of 100-200 bp. The site of length variation in the mouse germline corresponds to the location of these cloning-derived deletion events, suggesting that both phenomena are reflections of intrinsic genetic properties of these Ig gene 5' flanking sequences. |
