| First Author | Beach LR | Year | 1981 |
| Journal | Proc Natl Acad Sci U S A | Volume | 78 |
| Issue | 4 | Pages | 2110-4 |
| PubMed ID | 6941274 | Mgi Jnum | J:6523 |
| Mgi Id | MGI:54999 | Doi | 10.1073/pnas.78.4.2110 |
| Citation | Beach LR, et al. (1981) Amplification of the metallothionein-I gene in cadmium-resistant mouse cells. Proc Natl Acad Sci U S A 78(4):2110-4 |
| abstractText | Friend leukemia cells resistant to cadmium toxicity were selected. More than 70% of total cysteine incorporation in these cells was into the metal-binding protein, metallothionein. We used cDNA and genomic DNA clones containing the metallothionein-I gene to measure the concentration of its mRNA, the rate of gene transcription, and the number of genes. On a per cell basis, optimally induced, cadmium-resistant cells have a 14-fold more metallothionein-I mRNA, a 6-fold higher rate of metallothionein-I gene transcription, and 6-fold more metallothionein-I genes than do nonresistant cells. Metaphase spreads revealed that the resistant cells are nearly tetraploid and contain, on the average, three very small chromosomes that are absent from non-resistant Friend cells. |
