| First Author | Linzer DI | Year | 1983 |
| Journal | Proc Natl Acad Sci U S A | Volume | 80 |
| Issue | 14 | Pages | 4271-5 |
| PubMed ID | 6576337 | Mgi Jnum | J:32656 |
| Mgi Id | MGI:80146 | Doi | 10.1073/pnas.80.14.4271 |
| Citation | Linzer DI, et al. (1983) Growth-related changes in specific mRNAs of cultured mouse cells. Proc Natl Acad Sci U S A 80(14):4271-5 |
| abstractText | A cDNA plasmid library has been constructed from the poly(A)+ RNA present in BALB/c3T3 cells after serum stimulation. Of 3,500 clones tested, approximately 0.5% contained inserts corresponding to mRNAs present at higher levels in serum-stimulated BALB/c 3T3 cell cultures than in quiescent cultures. Most of these RNA species increased 2- to 5-fold, and the kinetics of increase for various RNAs differed. One clone (28H6) hybridized to a 1-kilobase RNA species that is present at barely detectable levels in resting cells but is increased at least 15- to 20-fold after serum stimulation, reaching a maximal level coincident with the onset of DNA synthesis. This RNA was at a high level in proliferating cells but decreased rapidly as cells reached confluence. 28H6 RNA was also increased in resting cells infected with simian virus 40 or stimulated with platelet-derived growth factor. |
