| First Author | Burt DW | Year | 1984 |
| Journal | Nucleic Acids Res | Volume | 12 |
| Issue | 22 | Pages | 8579-93 |
| PubMed ID | 6095203 | Mgi Jnum | J:7663 |
| Mgi Id | MGI:56132 | Doi | 10.1093/nar/12.22.8579 |
| Citation | Burt DW, et al. (1984) A retroviral provirus closely associated with the Ren-2 gene of DBA/2 mice. Nucleic Acids Res 12(22):8579-93 |
| abstractText | We have determined the entire nucleotide sequence of an intra-cisternal A particle (IAP) genome, associated with the Ren-2 gene of DBA/2 mice. This genome (MIARN) displays features common to other IAP retroviral-like genomes. Long terminal repeats (LTRs) are approximately 430 base pairs (bp) in length and show typical retroviral U3-R-U5 organisation, though the R-region, at 120 bp, is much larger than the average IAP. This difference probably arose by the amplification of a pyrimidine-rich sequence, by a slippage-mispairing mechanism. Flanking the 5' LTR is a sequence complementary to a phenylalanine tRNA, strongly conserved in all rodent IAP genomes and probably required to prime the initiation of (-) strand synthesis. Flanking the 3' LTR, is a purine-rich sequence probably required for (+) strand synthesis. The tRNA binding site (TBS) is flanked by six tandem copies of a sequence homologous to the TBS. The relationship of the MIARN element to other IAP genomes and the significance of its association with the highly expressed Ren-2 is discussed. |
