| First Author | Adamson ED | Year | 1985 |
| Journal | EMBO J | Volume | 4 |
| Issue | 4 | Pages | 941-7 |
| PubMed ID | 3894012 | Mgi Jnum | J:7929 |
| Mgi Id | MGI:56398 | Doi | 10.1002/j.1460-2075.1985.tb03722.x |
| Citation | Adamson ED, et al. (1985) Product of the cellular oncogene, c-fos, observed in mouse and human tissues using an antibody to a synthetic peptide. EMBO J 4(4):941-7 |
| abstractText | The transforming gene of the osteosarcoma-producing FBJ murine sarcoma virus, v-fos, is homologous to a normal cellular gene, c-fos, in vertebrate species. Transcripts from the c-fos proto-oncogene accumulate to very high levels in late gestational mouse and human extra-embryonic tissues. We now report that these RNA transcripts are translated in these tissues. Rabbits were immunized with a synthetic peptide whose sequence is common to both c-fos and v-fos. After affinity purification on an immunosorbent containing the fos peptide (a nonapeptide), the antibody reacted with a component(s) in nuclei in sections of human and murine tissues and immunoprecipitated the v-fos gene product (p55) and a cellular protein of 39 kd (p39, complexed with fos) from lysates of metabolically-labelled virally transformed cells. Crude extracts of normal tissues contained major anti-fos-reactive proteins in the range of 55-60 kd as shown by protein blot analysis. Indirect immunofluorescence and immunoperoxidase staining showed that in addition to strong immunoreactive component(s) in the nuclei of extra-embryonic tissues of human and mouse, weaker reactions are detectable in all normal fetal and adult tissues tested. This demonstrates that fos-reactive protein is expressed in a wide variety of cells and tissues. |
