| First Author | Mellor AL | Year | 1985 |
| Journal | Proc Natl Acad Sci U S A | Volume | 82 |
| Issue | 17 | Pages | 5920-4 |
| PubMed ID | 3862106 | Mgi Jnum | J:8005 |
| Mgi Id | MGI:56474 | Doi | 10.1073/pnas.82.17.5920 |
| Citation | Mellor AL, et al. (1985) Structure and expression of genes encoding murine Qa-2 class I antigens. Proc Natl Acad Sci U S A 82(17):5920-4 |
| abstractText | DNA structural analysis of the Qa region in two BALB/c mouse substrains with different Qa-2 phenotypes reveals that a deletion of DNA has occurred in BALB/cBy (Qa-2-) mice relative to BALB/c (Qa-2+) mice. We propose that this deletion arises from unequal crossing-over and recombination between adjacent BALB/c class I genes and results in the generation of a hybrid class I gene in BALB/cBy mice. Furthermore, we suggest that this is a direct cause of the change in Qa-2 phenotype. Further support for this model was obtained from transfection experiments in which cloned genes from the equivalent part of the Qa region in C57BL/10 mice were introduced into L cells. Four C57BL/10 genes, arranged in two almost identical pairs, encode polypeptides that are precipitated from lysates of transfectants with anti-Qa-2/3 antiserum. Although loss of one pair of these genes in BALB/c mice has no qualitative effect on Qa-2 phenotype, the loss of both pairs of genes via gene fusion leads to the loss of the Qa-2+ phenotype in BALB/cBy mice. |
