| First Author | Amar LC | Year | 1985 |
| Journal | EMBO J | Volume | 4 |
| Issue | 13B | Pages | 3695-700 |
| PubMed ID | 3004966 | Mgi Jnum | J:8194 |
| Mgi Id | MGI:56663 | Doi | 10.1002/j.1460-2075.1985.tb04137.x |
| Citation | Amar LC, et al. (1985) Mapping of the mouse X chromosome using random genomic probes and an interspecific mouse cross. EMBO J 4(13B):3695-700 |
| abstractText | Two libraries enriched in murine X chromosome material have been constructed in the lambda vector NM 1149 from flow-sorted chromosomes. Inserts of unique genomic sequence DNA were purified and their X chromosome specificity characterised by hybridisation to a panel of somatic cell hybrid lines. Of the first five such X chromosome-specific probes characterised, all detect restriction fragment length polymorphisms (RFLPs) between inbred mouse laboratory strains such as C57BL/6 and BALB/c and the SPE/Pas mouse strain established from a wild Mus spretus mouse, when their DNAs are digested with the restriction enzyme TaqI. Taking advantage of these RFLPs, all five probes have been localised on the X chromosome using an interspecific backcross between the B6CBARI and SPE/Pas mouse strains segregating the X chromosome markers hypoxanthine phosphoribosyl transferase (Hprt) and Tabby (Ta). Three of the probes map to the region between the centromere and Hprt, and two distal to Ta. Since such X-specific sequence probes detect RFLPs between M. spretus and M. musculus domesticus DNAs with high frequency, a large panel of well localised probes should soon be available for studies of biological problems associated with the X chromosome which can best be approached using the murine species. |
