First Author | Schuh R | Year | 1986 |
Journal | Proc Natl Acad Sci U S A | Volume | 83 |
Issue | 5 | Pages | 1364-8 |
PubMed ID | 2419906 | Mgi Jnum | J:8214 |
Mgi Id | MGI:56683 | Doi | 10.1073/pnas.83.5.1364 |
Citation | Schuh R, et al. (1986) Molecular cloning of the mouse cell adhesion molecule uvomorulin: cDNA contains a B1-related sequence. Proc Natl Acad Sci U S A 83(5):1364-8 |
abstractText | A clone (F20) containing coding sequences for the cell adhesion molecule uvomorulin was isolated by immunological techniques from cDNA library in the expression vector lambda gt11. The beta-galactosidase-uvomorulin fusion protein was used to affinity purify anti-uvomorulin antibodies. Affinity-purified antibodies recognized uvomorulin from cell lysates of embryonal carcinoma cells and reacted with the cell surface of embryonal carcinoma cells. The 1.8-kilobase cDNA insert hybridized to a single 4.3-kilobase poly(A)+ RNA species found only in cells expressing uvomorulin. Part of the nontranslated 3' sequences of the cloned uvomorulin cDNA is homologous to the interspersed B1 repeat of the mouse genome. |