| First Author | Boyd RT | Year | 1986 |
| Journal | Immunogenetics | Volume | 24 |
| Issue | 3 | Pages | 150-7 |
| PubMed ID | 2875941 | Mgi Jnum | J:8431 |
| Mgi Id | MGI:56898 | Doi | 10.1007/BF00364742 |
| Citation | Boyd RT, et al. (1986) Genetic polymorphism at the mouse immunoglobulin J kappa locus (Igk-J) as demonstrated by Southern hybridization and nucleotide sequence analysis. Immunogenetics 24(3):150-7 |
| abstractText | Comparison of the nucleotide sequences of the C.C58 M75 myeloma kappa chain gene and the BALB/c germ-line J kappa segments suggested that the J kappa regions of C.C58 and BALB/c might be distinguished by restriction enzyme polymorphisms. This was shown to be the case in Southern hybridizations of Hinf I and Acc I digests of liver DNA from these and other strains with a J kappa-specific probe. Tests of a wide variety of inbred, congenic, recombinant, and recombinant-inbred strains provided evidence for three alleles, Igk-Ja, Igk-Jb, and Igk-Jc, the type strains for which are C58/J, BALB/c, and SJL/J, respectively. Analysis of the B6.PL(85NS) congenic strain suggests that the Igk-J locus lies in the neighborhood of the Lyt-2/Lyt-3 loci, approximately 0.30 cM from the V gene segment determining the Igk-VSer and Igk-Efl polymorphisms. Finally, nucleotide substitutions lead to amino acid sequence differences between the C.C58 M 75 kappa gene and the BALB/c germ line in J kappa 2 and J kappa 4. Two of these substitutions reflect true germ-line differences, raising the possibility that idiotype differences observed among strains could reflect J kappa as well as V kappa differences. |
